Effect of different sterilization methods on the surface morphology of PPDO-hybrid-PLGA nanofiber scaffold and attachments of PC12 cell
Journal of the Korean Association of Oral and Maxillofacial Surgeons
;
: 635-639, 2008.
Artigo
em Coreano
| WPRIM
| ID: wpr-17453
ABSTRACT
OBJECTIVES:
the effect of different sterilization methods on the surface morphology of PPDO-hybrid-PLGA nanofiber scaffold and attachments of PC12 cell were investigated.METHODS:
Poly (p-dioxone)-hybrid-Poly (lactide-glycolide) (PPDO-hybrid-PLGA) nanofiber scaffold, fabricated in a tube form with 1.5 mm internal diameter, 0.2 mm thickness and 5 mm length, was prepared using electrospinning method. To study the surface morphology using SEM, The study group and control group in respective were; ControlNon-sterilized scaffold, Group Iscaffold sterilized with 70% Alcohol, Group II scaffold sterilized with Ethylene Oxide at 65 degrees C, and Group III scaffold sterilized with Ethylene Oxide at 37 degrees C. To investigate viability of the PC12 cell on the scaffold, The study group and control group in respective were; Control sterilized with 70% Alcohol, Group I sterilized with Ethylene Oxide at 65 degrees C, and Group II sterilized with Ethylene Oxide at 37 degrees C.RESULTS:
1. The surface morphology was slightly changed in Group I, II and GroupIII, compared with control. 2. The attachment of PC12 cells in Group I, II was not higher than in controlDISCUSSION:
The attachment of PC12 cell is not influenced by different sterilization methods.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Esterilização
/
Células PC12
/
Óxido de Etileno
/
Etilenos
/
Nanofibras
Limite:
Animais
Idioma:
Coreano
Revista:
Journal of the Korean Association of Oral and Maxillofacial Surgeons
Ano de publicação:
2008
Tipo de documento:
Artigo
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