SH3BP4, a novel pigmentation gene, is inversely regulated by miR-125b and MITF
Experimental & Molecular Medicine
;
: e367-2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-174861
ABSTRACT
Our previous work has identified miR-125b as a negative regulator of melanogenesis. However, the specific melanogenesis-related genes targeted by this miRNA had not been identified. In this study, we established a screening strategy involving three consecutive analytical approaches—analysis of target genes of miR-125b, expression correlation analysis between each target gene and representative pigmentary genes, and functional analysis of candidate genes related to melanogenesis—to discover melanogenesis-related genes targeted by miR-125b. Through these analyses, we identified SRC homology 3 domain-binding protein 4 (SH3BP4) as a novel pigmentation gene. In addition, by combining bioinformatics analysis and experimental validation, we demonstrated that SH3BP4 is a direct target of miR-125b. Finally, we found that SH3BP4 is transcriptionally regulated by microphthalmia-associated transcription factor as its direct target. These findings provide important insights into the roles of miRNAs and their targets in melanogenesis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pigmentação
/
Programas de Rastreamento
/
Biologia Computacional
/
MicroRNAs
/
Fator de Transcrição Associado à Microftalmia
Tipo de estudo:
Estudo prognóstico
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2017
Tipo de documento:
Artigo
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