Effect of Tumor Necrosis Factor-Alpha on Glomerular Epithelial Cells in Glomerular Permeability
Journal of the Korean Society of Pediatric Nephrology
;
: 1-9, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-174970
ABSTRACT
PURPOSE:
Minimal Change Disease (MCD) is the most common primary nephrotic syndrome in children. Some suggested that tumor necrosis factor-alpha (TNF-alpha) are involved in the pathogenesis of MCD.METHODS:
This study was done to see the changes of plasma and urinary TNF-alpha, and its effect on the determination of permeability of the glomerular basement membrane (BM) contributed by heparan sulfate proteoglycan (HSPG). Study patients consisted of 19 biopsy-proven MCD children aged 2-15 years old. Both plasma and urinary TNF-alpha were measured. Employing the Millicell system, TNF-alpha was screened for the permeability factors. We examined whether TNF-alpha regulated BM HSPG gene expression and HS synthesis in the glomerular epithelial cells (GECs).RESULTS:
Urinary TNF-alpha during relapse was significantly increased when compared with that of during remission or controls (364.4+/-51.2 vs 155.3+/-20.8, 36.0+/-4.5 ng/mg cr) (P< 0.05). However, negative results were obtained in the permeability assay using the Millicell system. No difference was seen in the BM HSPG gene expression and HS synthesis in the GECs.CONCLUSION:
It seems that TNF-alpha may not play a disease-specific role in the pathogenesis of MCD.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Permeabilidade
/
Plasma
/
Recidiva
/
Expressão Gênica
/
Fator de Necrose Tumoral alfa
/
Proteoglicanas de Heparan Sulfato
/
Células Epiteliais
/
Membrana Basal Glomerular
/
Nefrose Lipoide
/
Síndrome Nefrótica
Limite:
Criança
/
Humanos
Idioma:
Inglês
Revista:
Journal of the Korean Society of Pediatric Nephrology
Ano de publicação:
2004
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS