Cross-talk between Integrin and SMAD Signal Pathway in Articular Chondrocyte / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
;
: 65-75, 2007.
Artigo
em Coreano
| WPRIM
| ID: wpr-175138
ABSTRACT
PURPOSE:
Identifying the signal cross-talk between integrin signaling cascade and TGF-beta 1 signaling cascade in articular chondrocytes. MATERIALS ANDMETHODS:
To analyze integrin or TGF-beta 1 mediated signaling pathways from extracellular stimuli, type II collagen was coated on the cell culture plate and TGF-beta 1 was added to cell culture media. Chondrocytes were cultured in the conditioned media with each or both stimuli. Altered activation of signaling proteins detected with western blot technique.RESULTS:
More rapid attachment of cells was observed in the type II collagen coated group than non-coated group. The phosphorylated SMAD 2 and 3 were expressed in the type II collagen coated group and synergistically up-regulated phosphorylation in the co-treated group. The phosphorylated FAK at tyrosine 925 was activated by TGF-beta 1 treatment and synergistically up-regulated by both stimuli. But there was no meaningfully changed phosphorylation of extracellular signal regulated protein kinase (ERK) 1/2 and p38, as known downstream molecules of FAK cascade.CONCLUSION:
This result means that SMAD 2, SMAD 3 and tyrosine 925 of FAK are involved in this signal cross-talking in articular chondrocytes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosforilação
/
Proteínas Quinases
/
Tirosina
/
Transdução de Sinais
/
Western Blotting
/
Fator de Crescimento Transformador beta
/
Meios de Cultivo Condicionados
/
Técnicas de Cultura de Células
/
Condrócitos
/
Colágeno Tipo II
Tipo de estudo:
Estudo prognóstico
Idioma:
Coreano
Revista:
Journal of Korean Orthopaedic Research Society
Ano de publicação:
2007
Tipo de documento:
Artigo
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