Identification of Brucella abortus using the sequencing of omp gene / 대한내과학회지
Korean Journal of Medicine
; : 10-16, 2006.
Article
em Ko
| WPRIM
| ID: wpr-176114
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: As the incidence of bovine brucellosis increases in Korea, the incidence of human brucellosis is also increasing since 2002. However, it is difficult to identify Brucella species by using the conventional methods. METHODS: Three strains of gram-negative coccobacilli were isolated from blood specimens of three patients with prolonged fever, which were not identified by using the conventional methods. After extracting total DNA from these isolates, PCR amplification of 16S rRNA and omp2 genes was performed. These sequences secured by PCR assay were compared with known sequences by using GenBank BLAST. RESULTS: DNA sequences were obtained from 3 isolates by using PCR amplification of 16S rRNA. These sequences had more than 99.9% similarities with Brucella species by using GenBank BLAST. In the second place, after comparing DNA sequences secured by PCR amplification of omp2a and omp2b by using GenBank BLAST, these isolates were confirmed as B. abortus. CONCLUSIONS: DNA sequence analysis is a rapid and accurate method for identification of uncommon microorganisms, such as Brucella species.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Brucella
/
Brucella abortus
/
Brucelose
/
Brucelose Bovina
/
DNA
/
RNA Ribossômico 16S
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Incidência
/
Análise de Sequência de DNA
Tipo de estudo:
Diagnostic_studies
/
Incidence_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
País/Região como assunto:
Asia
Idioma:
Ko
Revista:
Korean Journal of Medicine
Ano de publicação:
2006
Tipo de documento:
Article