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Proteome Analysis of Differential Protein Expression in Cervical Cancer Cells after Paclitaxel Treatment / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment ; : 395-399, 2004.
Artigo em Inglês | WPRIM | ID: wpr-176922
ABSTRACT
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PURPOSE:

It is well known that infection with HPV (human papillomavirus) is the main cause of cervical cancer and certain types of HPV are recognized as carcinogens. At present, there is little information regarding the antineoplastic mechanism of paclitaxel against cervical carcinoma cells. We thus tried to analyze differential protein expression and antineoplastic mechanism-related proteins after paclitaxel treatment on cervical cancer cells by using a proteomic analysis and to investigate the mechanism of action. MATERIALS AND

METHODS:

Using proteomics analysis including 2-DE and MALDI-TOF-MS, we detected the antineoplastic mechanism-related proteins. Then, we performed western blot analysis for apoptosis- and transformation- related proteins to confirm expression patterns derived from proteome analysis after paclitaxel treatment.

RESULTS:

We identified several cellular proteins that are responsive to paclitaxel treatment in HeLa cells using proteomics methods. Paclitaxel treatment elevated main-ly apoptosis, immune response and cell cycle check point- related proteins. On the other hand, paclitaxel treatment diminished growth factor/oncogene-related proteins and transcription regulation-related proteins. Also, in the HPV-associated cervical carcinoma cells, paclitaxel demonstrated anti-proliferative activity through the membrane death receptor-mediated apoptotic pathway and the mitochondrial-mediated pathway.

CONCLUSION:

Identification and characterization of functionally modulated proteins involved in anti-cancer regulatory events should lead to a better nderstanding of the long-term actions of paclitaxel at the molecular level and will contribute to the future development of novel therapeutic drug treatments based upon current therapies.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Carcinógenos / Células HeLa / Ciclo Celular / Neoplasias do Colo do Útero / Western Blotting / Paclitaxel / Apoptose / Proteoma / Proteômica / Mãos Tipo de estudo: Estudo prognóstico Limite: Humanos Idioma: Inglês Revista: Cancer Research and Treatment Ano de publicação: 2004 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Carcinógenos / Células HeLa / Ciclo Celular / Neoplasias do Colo do Útero / Western Blotting / Paclitaxel / Apoptose / Proteoma / Proteômica / Mãos Tipo de estudo: Estudo prognóstico Limite: Humanos Idioma: Inglês Revista: Cancer Research and Treatment Ano de publicação: 2004 Tipo de documento: Artigo