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Effect of MITF-M and MITF-A Overexpression on the Dendrtic Formation in Melanocytes / 체질인류학회지
Korean Journal of Physical Anthropology ; : 27-34, 2016.
Artigo em Coreano | WPRIM | ID: wpr-17703
ABSTRACT
The microphthalmia-associated transcription factor (MITF), has been described as the master regulator of the basic helix-loop-helix leucine zipper family, involves melanogenesis in melanocytes. MITF consists of at least six isoforms, called MITF-M, MITF-A, MITF-B, MITF-C, MITF-H, and MITF-J. Previously, we found that not only MITF-M is expressed in the human hair follicle, but also MITF-A, MITF-C, MITF-H, and MITF-J isoforms are expressed in the skin. The aim of this study was to conform the MITF isoforms expressed in human skin, and investigate novel role of MITF isoforms in the melanocytes. Expression of MITF-M and MITF-A was found in primary melanoctyes and the melanoma cell lines. Interestingly, when MITF-M and MITF-A were overexpressed in the SK-MEL-24 melanoma cells by adenoviral transfection, length of the dendrites, serves as the principal conduit for melanosomes transfer, was significantly increased in the MITF-M overexpressed cells compared with the control group, and number of the dendtrites was significantly increased in the MITF-A overexpressed cells. A signal molecule involve in actin polymerization during dendrite formation, Rac1, was increased in the SK-MEL-24 melanoma cells treated with adenoviral MITF-M and MITF-A vectors. These results suggest that MITF-M and MITF-A induce dendrite formation via Rac1 signaling in the melanocytes.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Polímeros / Pele / Transfecção / Linhagem Celular / Zíper de Leucina / Actinas / Folículo Piloso / Melanossomas / Isoformas de Proteínas / Dendritos Limite: Humanos Idioma: Coreano Revista: Korean Journal of Physical Anthropology Ano de publicação: 2016 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Polímeros / Pele / Transfecção / Linhagem Celular / Zíper de Leucina / Actinas / Folículo Piloso / Melanossomas / Isoformas de Proteínas / Dendritos Limite: Humanos Idioma: Coreano Revista: Korean Journal of Physical Anthropology Ano de publicação: 2016 Tipo de documento: Artigo