Methods of Hematoxylin and Erosin Image Information Acquisition and Optimization in Confocal Microscopy / 대한의료정보학회지
Healthcare Informatics Research
;
: 238-242, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-177091
ABSTRACT
OBJECTIVES:
We produced hematoxylin and eosin (H&E) staining-like color images by using confocal laser scanning microscopy (CLSM), which can obtain the same or more information in comparison to conventional tissue staining.METHODS:
We improved images by using several image converting techniques, including morphological methods, color space conversion methods, and segmentation methods.RESULTS:
An image obtained after image processing showed coloring very similar to that in images produced by H&E staining, and it is advantageous to conduct analysis through fluorescent dye imaging and microscopy rather than analysis based on single microscopic imaging.CONCLUSIONS:
The colors used in CLSM are different from those seen in H&E staining, which is the method most widely used for pathologic diagnosis and is familiar to pathologists. Computer technology can facilitate the conversion of images by CLSM to be very similar to H&E staining images. We believe that the technique used in this study has great potential for application in clinical tissue analysis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Coloração e Rotulagem
/
Processamento de Imagem Assistida por Computador
/
Amarelo de Eosina-(YS)
/
Microscopia Confocal
/
Diagnóstico
/
Fluorescência
/
Hematoxilina
/
Métodos
/
Microscopia
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Healthcare Informatics Research
Ano de publicação:
2016
Tipo de documento:
Artigo
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