Development of an angiogenesis-focused cDNA chip and validation of its functionality
Experimental & Molecular Medicine
;
: 365-370, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-177635
ABSTRACT
DNA chip has been used as a powerful tool to study the genetic reprogramming of cells and its link to cellular phenotype such as angiogenesis. To evaluate the angiogenesis related genetic reprogramming more efficiently, we here developed an angiogenesis- focused cDNA chip containing 153 angiogenesis related genes arrayed in duplicate on a slide glass. In order to validate the functionality of the angiogenesis-focused cDNA chip, we examined gene expression profiles in HT1080 cells treated with either fetal bovine serum, a well known pro-angiogenic factor, or trichostatin A, a known angiogenesis inhibitor, using the cDNA chip. All duplicate data from the analysis are well matched with each other and gene expression profiles are well consistent with previously reported data. These results demonstrate that the angiogenesis-focused cDNA chip developed here can be a useful tool towards angiogenesisrelated researches.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células Tumorais Cultivadas
/
Expressão Gênica
/
Neovascularização Fisiológica
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Inibidores da Angiogênese
/
Perfilação da Expressão Gênica
/
Indutores da Angiogênese
/
Neovascularização Patológica
Limite:
Humanos
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2005
Tipo de documento:
Artigo
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