Increased Prooxidative Activity Stimulating the Oxidative Modification of Proteins in the Umbilical Venous Plasma and Placenta of Preeclampsia / 대한주산의학회잡지
Korean Journal of Perinatology
;
: 27-33, 2004.
Artigo
em Coreano
| WPRIM
| ID: wpr-178375
ABSTRACT
OBJECTIVE:
This study was performed to compare the prooxidative activity stimulating the protein carbonyl formation by cephalosporins in the umbilical venous and placenta of preeclampsia with that of normal pregnancy.METHODS:
Lipid peroxide levels in the umbilical venous plasma and placental tissue homogenates of normal pregnancy (n=12) and preeclampsia (n=12) were measured by thiobarbituric acid reaction. The basal protein carbonyl contents in the umbilical venous plasma and placental tissue homogenates of normal pregnancy (n=12) and preeclampsia (n=12) were determined by the 2,4-dinitrophenylhydrazine (DNPH) method. After samples of them were mixed and incubated up to 5 hours with 0.2 mL of 1 mM moxalactam or cephalothin, the protein carbonyl contents in them were measured by DNPH.RESULTS:
Protein carbonyls formation by moxalactam and cephalothin in the umbilical venous plasma and of women with preeclampsia were significantly higher than that of women with normal pregnancy (8.5+/-2.0 vs. 6.6+/-1.4 nmol/mg protein, p<0.05, 7.6+/-1.6 vs. 6.2+/-1.2 nmol/mg protein, p<0.05). Protein carbonyls formation by moxalactam and cephalothin in the placental tissue homogenates of women with preeclampsia were significantly higher than that of women with normal pregnancy (17.6+/-5.3 vs. 13.0+/-4.2 nmol/mg protein, p<0.05, 16.1+/-5.2 vs. 12.5+/-4.4 nmol/mg protein, p<0.05). There were significant positive correlations between lipid peroxide and cephalosporins induced protein carbonyls levels of umbilical venous plasma, and placental tissue homogenates (p<0.01).CONCLUSION:
These results suggest that increase in the prooxidative activity stimulating the oxidative modification of proteins in placenta may be involved in the pathogenesis of preecalmpsia.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Placenta
/
Plasma
/
Pré-Eclâmpsia
/
Cefalosporinas
/
Cefalotina
/
Carbonilação Proteica
/
Moxalactam
Limite:
Feminino
/
Humanos
/
Gravidez
Idioma:
Coreano
Revista:
Korean Journal of Perinatology
Ano de publicação:
2004
Tipo de documento:
Artigo
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