Biological Activities and Stability of a Standardized Pentacyclic Triterpene Enriched Centella asiatica Extract
Natural Product Sciences
;
: 20-24, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-178615
ABSTRACT
Pentacyclic triterpenes, mainly, asiatic acid, madecassic acid, asiaticoside, and madecassoside are the active constituents of Centella asiatica. A pentacyclic triterpene enriched C. asiatica extract (PRE) was prepared and standardized to contain a total pentacyclic triterpenes not less than 65% w/w. This work was focused on determination of antiinflammatory, antioxidant, and tyrosinase inhibitory activities of PRE and its stability. The PRE exhibited a satisfactory nitric oxide inhibitory effect, with an IC50 value of 64.6 µg/mL. In addition, the PRE inhibited tyrosinase enzyme activity with an IC50 value of 104.8 µg/mL. In contrast, the PRE possessed only weak antioxidant activity. The PRE was stable over a period of four months when stored as a dried powder but only in a well-closed container protected from light at 4 °C. An aqueous alcoholic solution of the PRE was stable at pH values of 5.8 and 7.0, but was not stable at a pH of 8.2. Preparations of the PRE in an aqueous solution should be performed in acidic or neutral conditions.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Monofenol Mono-Oxigenase
/
Concentração Inibidora 50
/
Centella
/
Triterpenos Pentacíclicos
/
Alcoólicos
/
Concentração de Íons de Hidrogênio
/
Óxido Nítrico
Limite:
Humanos
Idioma:
Inglês
Revista:
Natural Product Sciences
Ano de publicação:
2016
Tipo de documento:
Artigo
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