Study on the Detection of Chromosome X, Y Using in situ PCR / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 2568-2572, 1997.
Artigo
em Coreano
| WPRIM
| ID: wpr-179414
ABSTRACT
Untill now, classical karyotyping remains the most reliable and conclusive method, but sometimes very rapid and sensitive method for the detection of chromosomal abnormality is needed. The primed in situ labelling(PRINS) is very rapid and sensitive method for the detection of chromosomal aneuploidy and X-linked disorder instead of fluorescen in situ hybridization. The purpose of this study was to prepent the application of PRINS protocol to the rapid detection of chromosomes X and Y in both metaphases and interphase nuclei. The PRINS reaction was done in 10 amniocytes. We compared the PRINS result with conventional cytogenetic karyotyping. The result was the same with cytogenetic karyotyping. In the future, PRINS will become very useful and sensitive method for the chromosomal aneuploidy detection and X-linked disorder diagnosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Diagnóstico Pré-Natal
/
Reação em Cadeia da Polimerase
/
Aberrações Cromossômicas
/
Hibridização In Situ
/
Marcação in Situ com Primers
/
Citogenética
/
Diagnóstico
/
Interfase
/
Cariotipagem
/
Aneuploidia
Tipo de estudo:
Estudo diagnóstico
/
Guia de Prática Clínica
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Ano de publicação:
1997
Tipo de documento:
Artigo
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