In vitro inhibition of 10-formyltetrahydrofolate dehydrogenase activity by acetaldehyde
Nutrition Research and Practice
;
: 195-199, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-180634
ABSTRACT
Alcoholism has been associated with folate deficiency in humans and laboratory animals. Previous study showed that ethanol feeding reduces the dehydrogenase and hydrolase activity of 10-formyltetrahydrofolate dehydrogenase (FDH) in rat liver. Hepatic ethanol metabolism generates acetaldehyde and acetate. The mechanisms by which ethanol and its metabolites produce toxicity within the liver cells are unknown. We purified FDH from rat liver and investigated the effect of ethanol, acetaldehyde and acetate on the enzyme in vitro. Hepatic FDH activity was not reduced by ethanol or acetate directly. However, acetaldehyde was observed to reduce the dehydrogenase activity of FDH in a dose- and time-dependent manner with an apparent IC50 of 4 mM, while the hydrolase activity of FDH was not affected by acetaldehyde in vitro. These results suggest that the inhibition of hepatic FDH dehydrogenase activity induced by acetadehyde may play a role in ethanol toxicity.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oxirredutases
/
Leucovorina
/
Concentração Inibidora 50
/
Etanol
/
Alcoolismo
/
Ácido Fólico
/
Oxirredutases atuantes sobre Doadores de Grupo CH-NH
/
Fígado
/
Acetaldeído
/
Animais de Laboratório
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Nutrition Research and Practice
Ano de publicação:
2008
Tipo de documento:
Artigo
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