DNA Double Strand Breaks in Gastric Epithelium with Helicobacter pylori Infection / 대한소화기학회지
The Korean Journal of Gastroenterology
;
: 79-85, 2012.
Artigo
em Coreano
| WPRIM
| ID: wpr-180812
ABSTRACT
BACKGROUND/AIMS:
DNA double strand breaks (DSB) is one of the critical types of DNA damage. If unrepaired, DSB is accumulated in the nucleus of cells, the cells become apoptotic or transform to tumor by way of genomic instability. Some of malignant cancers and its premalignant lesions were proven to have DSB in their nuclei. There was no report that Helicobacter pylori (H. pylori), the gastric carcinogen, induce DNA DSB in gastric epithelium in vivo. The aim of this study was to investigate whether H. pylori induce DSB in the gastric epithelial cells of chronic gastritis.METHODS:
Immunohistochemical stains were performed for the DSB markers, phospho-53BP1 and gammaH2AX, in the gastric epithelium derived from 44 peptic ulcer disease patients before and after H. pylori eradication. DNA fragmentation assay was performed in the cell line to investigate the DNA damage by H. pylori infection.RESULTS:
The mean expression score of gammaH2AX was significantly higher in the H. pylori infected gastric epithelium as compared to the H. pylori eradicated gastric epithelium (8.8+/-5.5 vs. 6.2+/-5.3 respectively; p=0.008). The expression score of phospho-53BP1 between before and after eradication of H. pylori was not statistically different, but tended to be higher in H. pylori infection. DNA fragmentation was developed significantly more in the cell lines after infection with H. pylori.CONCLUSIONS:
DSB of DNA damage was typical feature of H. pylori infection in the gastric epithelium.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Úlcera Péptica
/
DNA
/
Imuno-Histoquímica
/
Histonas
/
Helicobacter pylori
/
Infecções por Helicobacter
/
Linhagem Celular Tumoral
/
Peptídeos e Proteínas de Sinalização Intracelular
/
Quebras de DNA de Cadeia Dupla
/
Mucosa Gástrica
Limite:
Adulto
/
Idoso
/
Aged80
/
Feminino
/
Humanos
/
Masculino
Idioma:
Coreano
Revista:
The Korean Journal of Gastroenterology
Ano de publicação:
2012
Tipo de documento:
Artigo
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