Magnesium Ascorbyl Phosphate Regulates the Expression of Inflammatory Biomarkers in Cultured Sebocytes
Annals of Dermatology
;
: 376-382, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-181208
ABSTRACT
BACKGROUND:
Acne is an inflammatory skin disorder caused by inflammatory biomarkers. Magnesium ascorbyl phosphate (MAP) is a stable precursor of vitamin C. It achieves a constant delivery of vitamin C into the skin and has antioxidative effects.OBJECTIVE:
We performed this study to evaluate the effect of MAP on the expression of inflammatory biomarkers in cultured sebocytes.METHODS:
Reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay were performed for inflammatory cytokines and matrix metalloproteinases (MMPs) before and after treatment of cultured sebocytes with MAP (10(-2) M), lipopolysaccharide (LPS) (5 microg/ml) and a combination of MAP and LPS. RT-PCR and western blotting were also performed for antimicrobial peptides (AMPs) and Toll-like receptor (TLR)-4 before and after treatment of cultured sebocytes with MAP, LPS, and a combination of MAP and LPS. Quantification of lipid peroxidation was also conducted.RESULTS:
The increased expression of inflammatory cytokines after treatment of cultured sebocytes with LPS was decreased after treatment with MAP. MMPs, AMPs, and TLR-4 were decreased after treatment of cultured sebocytes with MAP and a combination of MAP and LPS, and increased after treatment of cultured sebocytes with LPS alone. Lipid peroxidation was significantly decreased after treatment of cultured sebocytes with MAP and a combination of MAP and LPS. MAP decreased the increased lipid peroxidation after treatment of cultured sebocytes with LPS.CONCLUSION:
MAP may be an effective alternative agent to improve inflammatory reactions in acne.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peptídeos
/
Ácido Ascórbico
/
Pele
/
Ensaio de Imunoadsorção Enzimática
/
Biomarcadores
/
Peroxidação de Lipídeos
/
Lipopolissacarídeos
/
Western Blotting
/
Citocinas
/
Acne Vulgar
Idioma:
Inglês
Revista:
Annals of Dermatology
Ano de publicação:
2015
Tipo de documento:
Artigo
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