The Effects of Insulin Treatment on the Contractile Responses of the Seminal Vesicle in Streptozotocin-induced Diabetic Rats / 대한비뇨기과학회지

ABSTRACT

PURPOSE: Diabetes mellitus produces disturbances in reproductive function including decreased serum testosterone levels, however, the effects of diabetes on the contractile function of testosterone-dependent urogenital smooth muscles have not been conclusively established. In this study, the effects of streptozotocin-induced diabetes on the contractile responses of rat seminal vesicle were compared with those of insulin-treated diabetic and control groups. MATERIALS AND METHODS: Diabetes was induced by intraperitoneal injection of streptozotocin(65mg/kg) and in insulin-treated diabetic group, NPH was daily injected by subcutaneous route. All the rats were sacrificed by cervical dislocation after 6 weeks and the seminal vesicles were immediately removed. The changes of contractile response to electrical field stimulation, phenylephrine and KCI administration were measures by polygraph RESULTS: 1. The weights of seminal vesicle in streptozotocin-induced diabetes were significantly decreased than those of insulin-treated diabetic and control groups(p<0.05). 2. Contractile responses of seminal vesicle to electrical field stimulation were significantly decreased in streptozotocin-induced diabetic compared to insulin-treated diabetic and control groups(p<0.05). 3. Streptozotocin-induced diabetes produced significant increase in contractile responses of seminal vesicle to phenylephrine and KCI than insulin-treated diabetic and control groups(p<0.05). CONCLUSIONS: This study demonstrates that diabetes produce a denervation-like supersensitivity which is specific for receptor-mediated processes and that insulin treatment in diabetic mellitus is able to recover the contractile responses of seminal vesicle.