Effect of Antibiotic Suplementation of Balanced Salt Solution(BBS) Infusion During Cataract Surgery in vitro Experimental Model

ABSTRACT

We studied the effect of antibiotics (vancomycin(20microgram/ml) and gentamicin(8microgram/ml)) supplemented Balanced Salt Solution(BBS) infusion in reducing bacterial colonization during cataract surgery for an in-vitro eye model using sterilized Marty surgical simulator. We inoculated 107 logarithmic growth phase organisms of either gram(+), Staphylococcus aureus or gram(-), Pseudomonas aeruginosa into the anterior chamber prior to cataract surgery. Eyes using BSS infusion without antibiotic suplementation during cataract surgery served as controls. The contents of the anterior chamber were aspirated at the end of simulated cataract surgery and innoculated on appropriate culture media. The culture media were incubated at 37degrees C for 24hours and subsequently quantitative bacteriologic analysis was performed. There was no statistically significant difference between eyes which received antibiotic supplemented BSS infusion compared with eyes which received non-antibiotic supplemented BSS infusion at the end of the operation, but there was statistically significant difference between two groups 24 hours later(p<0.05).