E-cadherin and beta-catenin Expression and Mutation in Gastric Carcinomas
Journal of the Korean Gastric Cancer Association
; : 202-209, 2001.
Article
em Ko
| WPRIM
| ID: wpr-183102
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: When cancer cels invade the stroma, they should be dissociated from the adjacent cells at first. E-cadherin and beta-catenin constitute an important protein complex associated with cellular adhesion, development, and differentiation, especially in epithelial cells.The role of E-cadherin and beta- catenin in gastric carcinogenesis were studied. MATENRIALS AND METHODS: The expression of E-cadherin and beta-catenin in gastric adenocarcinomas by using immunohistochemical staining and the mutation by using polymerase chain reaction- single stranded conformation polymorphism (PCR-SSCP) and sequencing were performed in 40 adenocarcinomas and 5 dysplasia of stomach. Thirteen cases, which had lymph node metastasis, were also included for immunohistochemical staining. RESULTS: Inappropriate cytoplasmic and/or nuclear expression of a E-cadherin-beta-catenin complex was more frequent in poorly differentiated, diffuse type signet ring cell carcinomas than in well-differentiated, intestinal type adenocarcinomas (P<0.05). However, the expression was not related with clinical stage or lymph node metastasis. Mutation of E-cadherin was detected in 4 cases by using PCR-SSCP, whereas mutation of beta-catenin was detected in 2 cases. CONCLUSION: E-cadherin and beta-catenin seem to be important in gastric carcinogenesis, especially in poorly differentiated diffuse type.
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Índice:
WPRIM
Assunto principal:
Estômago
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Imuno-Histoquímica
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Adenocarcinoma
/
Caderinas
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Carcinoma de Células em Anel de Sinete
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Citoplasma
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Beta Catenina
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Carcinogênese
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Linfonodos
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Metástase Neoplásica
Idioma:
Ko
Revista:
Journal of the Korean Gastric Cancer Association
Ano de publicação:
2001
Tipo de documento:
Article