Production of Monoclonal Antibody to Polychlorinated Biphenyl Induced Cytochrome P-450 LMII in Rat Liver / 영남의대학술지
Yeungnam University Journal of Medicine
;
: 103-110, 1986.
Artigo
em Coreano
| WPRIM
| ID: wpr-184538
ABSTRACT
Cytochrome P-450 (CP-450) is one of the three components of the liver microsomal enzyme system which hydroxylates fatty acids, hydrocarbons and a variety of drugs and other foreign compounds. Female Balb/c mice were immunized with purified polychlorinated biphenyl (PCB) – induced CP-450 LMII. The spleen cells derived from immunized mice were fused with SP2 myeloma cells using polyethylene glycol (PEG 3500). The hybrid cells were selected by hypoxanthine-aminopterin-thymidine (HAT) medium and the culture fluid were screened by enzyme-linked immunosorbent assay to CP450 LMII. The hybrid cells(×107) were inoculated into intraperitoneal cavity of Balb/c mice for the purpose of production of ascetic fluids. Monoclonal antibody (Mab) was purified from ascitic fluid by DEAE cellulose ion exchange chromatography and I¹²⁵ labeled Mab was also confirmed by autoradiography and SDS-polyacrylamide gel electrophoresis (MW55,000 and 110,000)
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polietilenoglicóis
/
Autorradiografia
/
Baço
/
Líquido Ascítico
/
Ensaio de Imunoadsorção Enzimática
/
Cromatografia por Troca Iônica
/
Sistema Enzimático do Citocromo P-450
/
Citocromos
/
DEAE-Celulose
/
Eletroforese
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Coreano
Revista:
Yeungnam University Journal of Medicine
Ano de publicação:
1986
Tipo de documento:
Artigo
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