Bone Regeneration with MMP sensitive hyaluronic acid-based hydrogel, rhBMP-2 and nanoparticles in rat calvarial critical size defect(CSD) model
Journal of the Korean Association of Oral and Maxillofacial Surgeons
; : 137-145, 2009.
Article
em Ko
| WPRIM
| ID: wpr-185017
Biblioteca responsável:
WPRO
ABSTRACT
As an efficient controlled release system for rhBMP-2, a functional nanoparticle-hydrogel complex, incorporated with matrix metalloproteinase( MMP) sensitive peptide cross-linker, was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase(ALP) activity and mineral contents analysis, based on the rat calvarial critical size defect(8mm in diameter) model.Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded MMP sensitive hyaluronic acid(HA) based hydrogel- Nanoparticles(NP) complex, as compared to only MMP HA, the MMP HA-NP without rhBMP-2, or even with the rhBMP-2. These improvements included the formation pattern of bone and functional marrow, the degree of calcium quantification, and the ALP activity. These results indicate that the MMP sensitive HA with nano-particle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced rhBMP-2 scaffold.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Osteogênese
/
Medula Óssea
/
Regeneração Óssea
/
Cálcio
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Transplantes
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Hidrogéis
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Nanopartículas
/
Ácido Hialurônico
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
Ko
Revista:
Journal of the Korean Association of Oral and Maxillofacial Surgeons
Ano de publicação:
2009
Tipo de documento:
Article