Expression of Hyaluronidase-4 in a Rat Spinal Cord Hemisection Model
Asian Spine Journal
;
: 7-13, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-185086
ABSTRACT
STUDY DESIGN:
Examination of hyaluronidase-4 (Hyal-4) expression in a rat spinal cord hemisection model.PURPOSE:
To determine the status of Hyal-4 expression after hemisection of the spinal cord, and the relationship between its expression and that of chondroitin sulfate proteoglycans (CSPGs). OVERVIEW OF LITERATURE CSPGs are expressed at the site of spinal cord injury and inhibit axon regeneration. Administration of exogenous chrondroitinase ABC (ChABC), derived from bacteria, digested CSPGs and promoted axonal regrowth. Using a rat hemisection model, we have demonstrated peak CSPGs levels at by 3 weeks after injury but then decreased spontaneously. Could there be an endogenous enzyme similar to ChABC in the spinal cord? It has been suggested that Hyal-4 is involved in CSPG degradation.METHODS:
A rat hemisection model was prepared and spinal cord frozen sections were prepared at 4 days and 1, 2, 3, 4, 5, and 6 weeks post-cordotomy and stained for CSPGs and Hyal-4 and subjected to Western blotting.RESULTS:
CSPGs appeared at the injury site at 4 days after hemisection, reached a peak after 3 weeks, and then decreased. Hyal-4 was observed around the injury site from 4 days after cordotomy and increased until after 5-6 weeks. Double staining showed Hyal-4 around CSPGs. Western blotting identified a band corresponding to Hyal-4 from 4 days after hemisection.CONCLUSIONS:
Hyal-4 was expressed in a rat hemisection model in areas surrounding CSPGs, and as its peak was delayed compared with that of CSPGs. These results suggest the involvement of Hyal-4 in the digestion of CSPGs.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteoglicanas de Sulfatos de Condroitina
/
Regeneração
/
Medula Espinal
/
Traumatismos da Medula Espinal
/
Axônios
/
Bactérias
/
Western Blotting
/
Cordotomia
/
Digestão
/
Secções Congeladas
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Asian Spine Journal
Ano de publicação:
2015
Tipo de documento:
Artigo
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