Post-mortem re-cloning of a transgenic red fluorescent protein dog
Journal of Veterinary Science
;
: 405-407, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-186139
ABSTRACT
Recently, the world's first transgenic dogs were produced by somatic cell nuclear transfer. However, cellular senescence is a major limiting factor for producing more advanced transgenic dogs. To overcome this obstacle, we rejuvenated transgenic cells using a re-cloning technique. Fibroblasts from post-mortem red fluorescent protein (RFP) dog were reconstructed with in vivo matured oocytes and transferred into 10 surrogate dogs. One puppy was produced and confirmed as a re-cloned dog. Although the puppy was lost during birth, we successfully established a rejuvenated fibroblast cell line from this animal. The cell line was found to stably express RFP and is ready for additional genetic modification.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Baço
/
Traqueia
/
Animais Geneticamente Modificados
/
Regulação da Expressão Gênica
/
Clonagem de Organismos
/
Trato Gastrointestinal
/
Cães
/
Técnicas de Transferência Nuclear
/
Rim
/
Fígado
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2011
Tipo de documento:
Artigo
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