Evaluation of Two PCR-Hybridization Methods for the Detection of Mycobacterium tuberculosis / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 32-38, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-186850
ABSTRACT
BACKGROUND:
We evaluated two homemade polymerase chain reaction-hybridization (PCRH) assays for the detection of M. tuberculosis.METHODS:
Two PCRH assays, using a digoxigenin-luminescent probe (DL-PCRH) and using a radioactive probe (R-PCRH) were developed and compared. To determine the detection limit of each assay, 10-fold serial dilution samples of M. tuberculosis DNA were tested. To determine the specificity of each assay, 4 nontuberculous mycobacteria (NTM) and 13 bacteria other than mycobacteria were tested. Sputum samples from 50 patients with tuberculosis and 26 patients with nontuberculous diseases were tested by DL-PCRH, R-PCRH, culture, AFB stain and PCR assay methods, respectively.RESULTS:
Both of the DL-PCRH and R-PCRH methods showed the same detection limit of 100 fg of M. tuberculosis DNA which was a log higher than that for the PCR. Sensitivity of both methods for the detection of M. tuberculosis in sputum specimens was 84%, which were slightly inferior to that of the culture (90%) and similar to that of the PCR (82%). However, both DL-PCRH and R-PCRH methods correctly identified M. tuberculosis for all six specimens that showed weakly-positive or negative PCR results. No false-positive results were obtained for patients with nontuberculous diseases (n=26), NTM (n=4) and other bacteria (n=13).CONCLUSIONS:
Although the diagnostic efficiency of the two PCRH assays were similar to that of the PCR, both methods have greater objectivity in reading results than the PCR. This suggests that the PCRH is suitable for use in large number of clinical samples for the rapid detection of M. tuberculosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Escarro
/
Bactérias
/
Tuberculose
/
DNA
/
Reação em Cadeia da Polimerase
/
Sensibilidade e Especificidade
/
Limite de Detecção
/
Micobactérias não Tuberculosas
/
Mycobacterium tuberculosis
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Coreano
Revista:
The Korean Journal of Laboratory Medicine
Ano de publicação:
2003
Tipo de documento:
Artigo
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