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Effect of Multidrug Resistance Gene-1 (mdr1) Overexpression on In-Vitro Uptake of 99mTc-sestaMIBI in Murine L1210 Leukemia Cells / 대한핵의학회잡지
Korean Journal of Nuclear Medicine ; : 152-162, 1999.
Artigo em Coreano | WPRIM | ID: wpr-186941
ABSTRACT

PURPOSE:

To determine whether Tc-99mMIBI is recognized by the multidrug resistant P-glycoprotein (Pgp), we have measured quantitatively Tc-99mMIBI uptake in cancer cells. The effects of various Pgp reversing agents on cellular Tc-99m-MIBI uptake were also investigated in the presence of multidrug resistance gene-1 (mdr1 gene) overexpression. MATERIALS AND

METHODS:

We measured percentage uptake of Tc-99m-MIBI at different incubation temperatures both in mdr1 positive and negative cells. The effects of verapamil, cyclosporin, and dipyridamole on cellular uptake of Tc-99m-MIBI were also evaluated with or without overexpression of mdr1 gene in cultured murine leukemia L1210 cells.

RESULTS:

The mdr1 gene expressing cell lines were effectively induced in in vitro with continuous application of low-dose adriamycin or vincristine. Cellular uptake of Tc-99m-MIBI was higher in mdr1 negative L1210 cells than those of mdr1 positive cells, and higher when incubated in 37 degree C than 4 degree C. In the presence of verapamil, cyclosporin or dipyridamole, Tc-99m-MIBI uptake was increased upto 604% in mdr1 positive cells.

CONCLUSION:

Cellular uptake of Tc-99m-MIBI is lower in leukemia cells over-expressing mdr1 gene, and MDR-reversing agents increase cellular uptake. These results suggest that Tc-99m-MIBI can be used for characterizing Pgp expression and developing MDR-reversing agents In vitro.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Vincristina / Verapamil / Leucemia / Leucemia L1210 / Doxorrubicina / Linhagem Celular / Ciclosporina / Tecnécio Tc 99m Sestamibi / Membro 1 da Subfamília B de Cassetes de Ligação de ATP / Resistência a Múltiplos Medicamentos Limite: Animais Idioma: Coreano Revista: Korean Journal of Nuclear Medicine Ano de publicação: 1999 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Vincristina / Verapamil / Leucemia / Leucemia L1210 / Doxorrubicina / Linhagem Celular / Ciclosporina / Tecnécio Tc 99m Sestamibi / Membro 1 da Subfamília B de Cassetes de Ligação de ATP / Resistência a Múltiplos Medicamentos Limite: Animais Idioma: Coreano Revista: Korean Journal of Nuclear Medicine Ano de publicação: 1999 Tipo de documento: Artigo