Effect of ABT-594 a Selective Nicotinic Agonist, on Voiding Function in Spinal Cord Injury Rat

ABSTRACT

PURPOSE: In this study we demonstrate effect of selective nicotinic agonist, ABT-594 on voiding in spinal cord injury rat. MATERIALS AND METHODS: Spinal cord injury rat was made by complete resection of the spinal cord(T8~9) and checked cystometry three weeks after injury. In female Sprague-Dawley awake rats, an intravesical catheter was inserted through the bladder dome and intravenous catheter was inserted to right jugular vein. After the surgery, cystometry was performed by infusing saline into the bladder(0.08 ml/min). Cumulative doses of ABT-594 (0.01, 0.1, 1, 10 ug/kg) were injected intravenously in spinal cord injury rat at 1 hour intervals. RESULTS: All dose(0.01~10 ug/kg) of ABT-594 did not affect the intercontraction interval(ICI), maximal voiding pressure(MVP), and pressure threshold(PT), and from voiding contraction to non-voiding contraction(from VC to NVC), voiding volume(VV), residual volume(RV). But moderate dose(1 ug) of ABT-594 significantly decreased number of non-voiding contraction(55.6+/-13.7% of control)(p<0.05). CONCLUSION: These result suggest that the nicotinic agonist ABT-594 might in part affect voiding function through stimulation of serotonergic nerve to lumbosacral level in spinal cord injury rat.