Detection of chromosome aberrations in interphase nuclei using fluorescence in situ hybridization technique
Journal of Korean Medical Science
;
: 257-261, 1993.
Artigo
em Inglês
| WPRIM
| ID: wpr-18734
ABSTRACT
We report here several experiences of interphase cytogenetics, using fluorescence in situ hybridization (FISH) technique, for the detection of chromosome aberrations. FISH, using alpha satellite specific probes of 18, X, Y chromosomes, was done in interphase nuclei from peripheral blood of patients with Edwards' syndrome, Klinefelter's syndrome and Turner's syndrome with healthy male and female controls, respectively. The distributions of fluorescent signals in 100 interphase nuclei were well correlated with metaphase findings. Nowadays FISH plays an increasingly important role in a variety of research areas, including cytogenetics, prenatal diagnosis, tumor biology, gene amplification and gene mapping.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Núcleo Celular
/
Aberrações Cromossômicas
/
Bandeamento Cromossômico
/
Hibridização in Situ Fluorescente
/
Interfase
Tipo de estudo:
Estudo diagnóstico
Limite:
Adolescente
/
Adulto
/
Criança
/
Feminino
/
Humanos
/
Masculino
/
Recém-Nascido
Idioma:
Inglês
Revista:
Journal of Korean Medical Science
Ano de publicação:
1993
Tipo de documento:
Artigo
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