IDH Mutation Analysis in Ewing Sarcoma Family Tumors
Journal of Pathology and Translational Medicine
;
: 257-261, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-188224
ABSTRACT
BACKGROUND:
Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to yield alpha-ketoglutarate (alpha-KG) with production of reduced nicotinamide adenine dinucleotide (NADH). Dysfunctional IDH leads to reduced production of alpha-KG and NADH and increased production of 2-hydroxyglutarate, an oncometabolite. This results in increased oxidative damage and stabilization of hypoxia-inducible factor alpha, causing cells to be prone to tumorigenesis.METHODS:
This study investigated IDH mutations in 61 Ewing sarcoma family tumors (ESFTs), using a pentose nucleic acid clamping method and direct sequencing.RESULTS:
We identified four cases of ESFTs harboring IDH mutations. The number of IDH1 and IDH2 mutations was equal and the subtype of IDH mutations was variable. Clinicopathologic analysis according to IDH mutation status did not reveal significant results.CONCLUSIONS:
This study is the first to report IDH mutations in ESFTs. The results indicate that ESFTs can harbor IDH mutations in previously known hot-spot regions, although their incidence is rare. Further validation with a larger case-based study would establish more reliable and significant data on prevalence rate and the biological significance of IDH mutations in ESFTs.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sarcoma de Ewing
/
Incidência
/
Prevalência
/
Constrição
/
Descarboxilação
/
Carcinogênese
/
Isocitrato Desidrogenase
/
NAD
Tipo de estudo:
Estudo de incidência
/
Estudo de prevalência
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Pathology and Translational Medicine
Ano de publicação:
2015
Tipo de documento:
Artigo
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