Surface characteristics and osteoblastic cell response of alkali-and heat-treated titanium-8tantalum-3niobium alloy
Journal of Periodontal & Implant Science
; : 248-255, 2012.
Article
em En
| WPRIM
| ID: wpr-188637
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: The aim of the present study was to evaluate the biological response of alkali- and heat-treated titanium-8tantalum-3niobium surfaces by cell proliferation and alkaline phosphatase (ALP) activity analysis. METHODS: Commercial pure titanium (group cp-Ti) and alkali- and heat-treated titanium-8tantalum-3niobium (group AHT) disks were prepared. The surface properties were evaluated using scanning electron microscopy, energy dispersed spectroscopy and X-ray photoelectron spectroscopy (XPS). The surface roughness was evaluated by atomic force microscopy and a profilometer. The contact angle and surface energy were also analyzed. The biological response of fetal rat calvarial cells on group AHT was assessed by cell proliferation and ALP activity. RESULTS: Group AHT showed a flake-like morphology microprofile and dense structure. XPS analysis of group AHT showed an increased amount of oxygen in the basic hydroxyl residue of titanium hydroxide groups compared with group cp-Ti. The surface roughness (Ra) measured by a profilometer showed no significant difference (P>0.05). Group AHT showed a lower contact angle and higher surface energy than group cp-Ti. Cell proliferation on group AHT surfaces was significantly higher than on group cp-Ti surfaces (P<0.05). In comparison to group cp-Ti, group AHT enhanced ALP activity (P<0.05). CONCLUSIONS: These results suggest that group AHT stimulates osteoblast differentiation.
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Índice:
WPRIM
Assunto principal:
Osteoblastos
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Oxigênio
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Análise Espectral
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Propriedades de Superfície
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Titânio
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Microscopia Eletrônica de Varredura
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Adesão Celular
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Microscopia de Força Atômica
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Proliferação de Células
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Fosfatase Alcalina
Limite:
Animals
Idioma:
En
Revista:
Journal of Periodontal & Implant Science
Ano de publicação:
2012
Tipo de documento:
Article