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Expression of surface markers and myogenic potential of rat bone marrow- and adipose-derived stem cells: a comparative study / 대한해부학회지
Anatomy & Cell Biology ; : 113-121, 2013.
Artigo em Inglês | WPRIM | ID: wpr-188660
ABSTRACT
In recent years, examination and comparison of the biological characteristics of bone marrow- and adipose-derived mesenchymal stem cells (MSCs) from various perspectives have come into the focus of stem cell research, as these cells should be well characterized in order to utilize them in future cellular therapies. Therefore, in the present study, surface protein markers and the skeletal myogenic differentiation potential of rat bone marrow- and adipose-derived MSCs were examined. The expression of CD44, CD45, CD73, and CD90 on bone marrow- and adipose-derived MSCs was characterized using flow cytometry. Subsequently, the stem cells were differentiated into myogenic lineages, and the expression of the skeletal myogenic markers MyoD1, Myog, and Myh2 was studied in cells using real time polymerase chain reaction and immunofluorescence. Our results reveal that the pattern of CD marker expression differs between these 2 types of MSCs to some extent, whereas no significant difference was observed with respect to their myogenic differentiation potential. Therefore, we concluded that despite the differences observed in the biological features of these 2 types of MSCs, their myogenic potential appears to be similar, and that adipose-derived stem cells may be useful in skeletal muscle tissue engineering, due to their easy isolation and capacity for rapid expansion in a short time span.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Células-Tronco / Características da População / Imunofluorescência / Músculo Esquelético / Engenharia Tecidual / Pesquisa com Células-Tronco / Células-Tronco Mesenquimais / Reação em Cadeia da Polimerase em Tempo Real / Citometria de Fluxo / Antígenos de Superfície Limite: Animais Idioma: Inglês Revista: Anatomy & Cell Biology Ano de publicação: 2013 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Células-Tronco / Características da População / Imunofluorescência / Músculo Esquelético / Engenharia Tecidual / Pesquisa com Células-Tronco / Células-Tronco Mesenquimais / Reação em Cadeia da Polimerase em Tempo Real / Citometria de Fluxo / Antígenos de Superfície Limite: Animais Idioma: Inglês Revista: Anatomy & Cell Biology Ano de publicação: 2013 Tipo de documento: Artigo