Influence of Transforming Growth Factor-alpha on Expression of Matrix metalloproteinase-2 and Matrix metalloproteinase-9 mRNA in Mouse Embryos / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 443-449, 2002.
Artigo
em Coreano
| WPRIM
| ID: wpr-188992
ABSTRACT
OBJECTIVE:
To investigate the influence of transforming growth factor-alpha (TGF-alpha) on the expression of Matrix metalloproteinase-2 (MMP-2) and Matrix metalloproteinase-9 (MMP-9) mRNA in mouse embryos. MATERIALS ANDMETHOD:
Eight-cell stage mouse embryos were cultured for 48hours with TGF-alpha at concentrations of 1, 10 and 100 ng/ml. Embryos not treated with TGF-alpha served as control. Reverse transcription-polymerase chain reaction (RT-PCR) has been used to examine the expression of MMP-2 and MMP-9 mRNA in developed blastocysts. Following reverse transcription, strategically designed nested primers, optimized for specificity, were used for amplification from the cDNA equivalent of a single embryo. The products were then verified by restriction enzyme digestion and sequence analysis. Results were analyzed with analysis of variance (ANOVA) and statistical significance was defined as p<0.05.RESULTS:
The relative quantities (relative volume x intensity) of MMP-2 mRNA expressed in embryos of 10 and 100 ng/m of TGF-alpha treatment groups were significantly increased than in the control and 1 ng/ml of TGF-alpha treatment group (67.2+/-7.5 and 77.4+/-11.6 vs. 38.6+/-4.5 and 43.4+/-6.1, p<0.001). The relative quantities of MMP-9 mRNA of 100 ng/ml TGF-alpha treatment group was significantly increased than in the control and 1 ng/ml TGF-alpha treatment groups (67.6+/-6.5 vs. 36.6+/-14.2 and 40.2+/-11.3, p<0.001, p<0.01, respectively).CONCLUSION:
This study suggests that TGF-alpha itself may induce the expression of MMP-2 and 9 mRNA in mouse embryos.
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Blastocisto
/
RNA Mensageiro
/
Fator de Crescimento Transformador alfa
/
Sensibilidade e Especificidade
/
Análise de Sequência
/
DNA Complementar
/
Metaloproteinase 2 da Matriz
/
Metaloproteinase 9 da Matriz
/
Transcrição Reversa
/
Digestão
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Ano de publicação:
2002
Tipo de documento:
Artigo
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