Comparison of bromodeoxyuridine and proliferating cell nuclear antigen labeling in gastric carcinoma
Journal of Korean Medical Science
;
: 16-20, 1994.
Artigo
em Inglês
| WPRIM
| ID: wpr-189276
ABSTRACT
The proliferative activity of human gastric carcinoma was measured by means of in vitro incorporation of the thymidine analogue, 5-bromo-2'-deoxyuridine (BrdU), into the newly-synthesized DNA of fresh tumors and immunohistochemical staining of proliferating cell nuclear antigen (PCNA) using avidin-biotin peroxidase method. Eighty-two cases of surgically resected human gastric carcinomas consisting of 18 various histologic types were subjected to study. The mean BrdU labelling index (LI) and PCNA LI were 22.9% and 39.1%, respectively. The correlation between BrdU LI and PCNA LI was statistically significant (correlation coefficient mu = 0.61334, p = 0.0001). We concluded that immunohistochemical staining for PCNA may become a practical method instead of in vitro or in vivo BrdU labeling to assess the proliferation fraction of the gastric cancer patient.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Gástricas
/
Bromodesoxiuridina
/
DNA de Neoplasias
/
Proteínas Nucleares
/
Células Tumorais Cultivadas
/
Carcinoma
/
Divisão Celular
/
Técnicas Imunoenzimáticas
/
Antígeno Nuclear de Célula em Proliferação
/
Replicação do DNA
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Korean Medical Science
Ano de publicação:
1994
Tipo de documento:
Artigo
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