Phylogenetic Relationships of 3 Korean Neodiplostomum Species (Digenea: Neodiplostomidae) Based on Partial CO1 Gene
The Korean Journal of Parasitology
;
: 325-329, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-190458
ABSTRACT
The phylogenetic relationships of the 3 Neodiplostomum spp. (Digenea Neodiplostomidae) occurring in Korea (N. seoulense, N. leei, and N. boryongense) were analyzed using the partial mitochondrial cytochrome c oxidase subunit 1 (CO1) gene. The adult flukes were recovered from Sprague-Dawley rats (N. seoulense) and newborn chicks (N. leei and N. boryongense) experimentally infected with the neodiplostomula from the grass snake, Rhabdophis tigrinus tigrinus. The genomic DNA was amplified using specific primers, and the sequence of CO1 was obtained. According to the results, the pairwise similarity was 96.1% between N. boryongense and N. seoulense, but was 95.0% between N. boryongense and N. leei and 94.2% between N. leei and N. seoulense. The results demonstrated a closer phylogenetic relationship between N. seoulense and N. boryongense. This high relationship of N. seoulense and N. boryongense may be related to their similar morphologic features including the limited distribution of vitellaria and the presence of a genital cone. N. leei is distinct on the other hand with an extensive distribution of vitellaria and the absence of a genital cone.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Trematódeos
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Homologia de Sequência do Ácido Nucleico
/
Análise por Conglomerados
/
Galinhas
/
Análise de Sequência de DNA
/
Ratos Sprague-Dawley
/
Colubridae
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2014
Tipo de documento:
Artigo
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