Detection of Enterovirus in Cerebrospinal Fluid by Real-Time Nested Reverse Transcription Polymerase Chain Reaction / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 9-13, 2006.
Artigo
em Coreano
| WPRIM
| ID: wpr-190587
ABSTRACT
BACKGROUND:
Enterovirus is a common cause of aseptic meningitis, respiratory disease and nonspecific febrile illness. The conventional methods for laboratory diagnosis of enterovirus infections have been virus culture and serotyping by an immunofluorecent test. We studied a new and more rapid approach for enterovirus detection in cerebrospinal fluid (CSF) by real-time nested PCR.METHODS:
This study was performed on 50 CSF specimens from patients suspected of aseptic meningitis. Enterovirus was detected in CSF by PCRs for 3 different targets and real-time nested PCR. Enterovirus culture was also performed in 44 CSF specimens.RESULTS:
The positive rate of PCRs for each of the 3 different targets was 26.0%, 40.0%, or 46.0%, and that of real-time nested PCR was 86.0%. Only 6.8% were positive in culture. Thus, the positive rate of real-time nested PCR was much higher than other methods.CONCLUSIONS:
Our study revealed that the real-time nested PCR should be useful for diagnosis of enterovirus infections because of a high sensitivity and rapid detection.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sorotipagem
/
Líquido Cefalorraquidiano
/
Reação em Cadeia da Polimerase
/
Enterovirus
/
Técnicas de Laboratório Clínico
/
Transcrição Reversa
/
Diagnóstico
/
Infecções por Enterovirus
/
Reação em Cadeia da Polimerase em Tempo Real
/
Meningite Asséptica
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Coreano
Revista:
The Korean Journal of Laboratory Medicine
Ano de publicação:
2006
Tipo de documento:
Artigo
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