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Modification of adriamycin-induced cytotoxicity by recombinant human interferon-gamma and/or verapamil in human stomach cancer cells
Journal of Korean Medical Science ; : 236-240, 1992.
Artigo em Inglês | WPRIM | ID: wpr-191169
ABSTRACT
Recombinant human-interferon-gamma (rH-IFN-gamma) and verapamil (VRP), either alone or in combination, were evaluated in MTT assay for their modification effects on adriamycin-induced cytotoxicity against MKN-45, human stomach adenocarcinoma cells. VRP as a single agent did not inhibit the survival of MKN-45 at doses of up to 5.0 micrograms/ml. The survival of MKN-45 was inhibited by rH-IFN-gamma dose-dependently and further inhibited by the addition of VRP. However, the maximum growth inhibition of MKN-45 in any combination treatment with rH-IFN-gamma and VRP was less than 50% except in the highest concentration combinations (% survival 47.9% at 10(4) U/ml of rH-IFN-gamma and 3.0 micrograms/ml of VRP). Adriamycin caused a concentration-dependent cytotoxicity and its cytotoxicity was significantly enhanced by the addition of rH-IFN-gamma and further enhanced by the combined use of rH-IFN-gamma and VRP. The modification effects of rH-IFN-gamma and VRP on adriamycin-induced cytotoxicity were evaluated in terms of modification index (MI), demonstrating that rH-IFN-gamma significantly increased in adriamycin-induced cytotoxicity and that the combined use of rH-IFN-gamma and VRP enhanced the adriamycin-induced cytotoxicity to a greater extent than did rH-IFN-gamma alone MI values at 10(2) U/ml and 10(3) U/ml of rH-IFN-gamma were 1.7 and 3.1, respectively; those at 1.5 micrograms/ml and 3.0 micrograms/ml of VRP in the presence of 10(3) U/ml of rH-IFN-gamma were 4.4 and 6.0, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Neoplasias Gástricas / Proteínas Recombinantes / Resistência a Medicamentos / Ensaios de Seleção de Medicamentos Antitumorais / Células Tumorais Cultivadas / Verapamil / Adenocarcinoma / Protocolos de Quimioterapia Combinada Antineoplásica / Doxorrubicina / Sobrevivência Celular Limite: Humanos Idioma: Inglês Revista: Journal of Korean Medical Science Ano de publicação: 1992 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Neoplasias Gástricas / Proteínas Recombinantes / Resistência a Medicamentos / Ensaios de Seleção de Medicamentos Antitumorais / Células Tumorais Cultivadas / Verapamil / Adenocarcinoma / Protocolos de Quimioterapia Combinada Antineoplásica / Doxorrubicina / Sobrevivência Celular Limite: Humanos Idioma: Inglês Revista: Journal of Korean Medical Science Ano de publicação: 1992 Tipo de documento: Artigo