Effective glycemic control achieved by transplanting non-viral cationic liposome-mediated VEGF-transfected islets in streptozotocin-induced diabetic mice
Experimental & Molecular Medicine
; : 513-523, 2005.
Article
em En
| WPRIM
| ID: wpr-191500
Biblioteca responsável:
WPRO
ABSTRACT
Hypoxic damage is one of the major causes of islet graft failure and VEGF is known to play a crucial role in revascularization. To address the effectiveness of a cationic lipid reagent as a VEGF gene carrier, and the beneficial effect of VEGF-transfected islets on glycemic control, we used effectene lipid reagent in a transfection experiment using mouse islets. Transfection efficiencies were highest for 4 microgram/microliter cDNA and 25 microliter effectene and cell viabilities were also satisfactory under this condition, and the overproduction of VEGF mRNA and protein were confirmed from conditioned cells. A minimal number of VEGF-transfected islets (100 IEQ/animal) were transplanted into streptozotocin (STZ)-induced diabetic mice. Hyperglycemia was not controlled in the islet transplantation (IT)-alone group (0/8) (non- diabetic glucose mice number/total recipient mice number) or in the IT-pJDK control vector group (0/8). However, hyperglycemia was completely abrogated in the IT-pJDK-VEGF transduced group (8/8), and viable islets and increased VEGF-transfected grafts vascularization were observed in renal capsules.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Peso Corporal
/
RNA Mensageiro
/
Transfecção
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Sobrevivência Celular
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Transplante das Ilhotas Pancreáticas
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Ilhotas Pancreáticas
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Estreptozocina
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Neovascularização Fisiológica
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Fatores de Crescimento do Endotélio Vascular
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Diabetes Mellitus Experimental
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2005
Tipo de documento:
Article