Evaluation of alpha-Tubulin as an Antigenic and Molecular Probe to Detect Giardia lamblia
The Korean Journal of Parasitology
;
: 287-291, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-191532
ABSTRACT
The alpha/beta-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant alpha-tubulin of Giardia lamblia were made, and found effective as a probe to specifically detect G. lamblia by immunofluorescence assays. Nucleotide sequences of alpha-tubulin genes were compared between G. lamblia WB and GS strains, prototypes of assemblage A and assemblage B, respectively. A set of primers was designed and used to amplify a portion of the alpha-tubulin gene from G. lamblia. PCR-RFLP analysis of this alpha-tubulin PCR product successfully differentiated G. lamblia into 2 distinct groups, assemblages A and B. The results indicate that alpha-tubulin can be used as a molecular probe to detect G. lamblia.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tubulina (Proteína)
/
Polimorfismo de Fragmento de Restrição
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Sondas Moleculares
/
Proteínas de Protozoários
/
Reação em Cadeia da Polimerase
/
Alinhamento de Sequência
/
Giardíase
/
Giardia lamblia
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2009
Tipo de documento:
Artigo
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