The Relationship of a Combination of Human Adipose Tissue-Derived Stem Cells and Frozen Fat with the Survival Rate of Transplanted Fat
Archives of Plastic Surgery
;
: 677-685, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-192169
ABSTRACT
BACKGROUND:
The survival rate of grafted fat is difficult to predict, and repeated procedures are frequently required. In this study, the effects of the freezing period of harvested adipose tissue and the addition of human adipose tissue-derived stem cells (ASCs) on the process of fat absorption were studied.METHODS:
Adipose tissue was obtained from patients who underwent a lipoaspirated fat graft. The fat tissue was cryopreserved at -20degrees C in a domestic refrigerator. A total of 40 nude mice were used. The mice in the experimental group received three different subcutaneous injections in the back an injection of fresh fat and ASCs, an injection of fat that had been frozen for one month and ASCs, and an injection of fat that had been frozen for two months and ASCs. The control mice received fat grafts without ASCs. The mice were sacrificed at four or eight weeks after the procedure, and the grafted fat tissues were harvested. The extracted fat was evaluated using photographic analysis, volume measurements, and histological examination.RESULTS:
In the control group, the fat resorption rates four weeks after transplantation in the grafts of fresh fat, fat that had been frozen for one month, and fat that had been frozen for two months were 21.14%, 22.46%, and 42.56%, respectively. In the experimental group, the corresponding resorption rates were 6.68%, 13.0%, and 33.9%, respectively.CONCLUSIONS:
ASCs can increase the fat graft survival rate. The use of ASCs in fat grafting can reduce the need for repeated fat grafts and provide good long term results.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células-Tronco
/
Criopreservação
/
Tecido Adiposo
/
Taxa de Sobrevida
/
Transplantes
/
Absorção
/
Congelamento
/
Sobrevivência de Enxerto
/
Injeções Subcutâneas
/
Camundongos Nus
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Archives of Plastic Surgery
Ano de publicação:
2015
Tipo de documento:
Artigo
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