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Recombinant influenza viruses as delivery vectors for hepatis B virus epitopes
Clinical and Experimental Vaccine Research ; : 77-82, 2012.
Artigo em Inglês | WPRIM | ID: wpr-192289
ABSTRACT

PURPOSE:

Neuraminidase (NA) of influenza virus contains stalk region that shows a great deal of variability in both amino acid sequence and length. In this paper, we investigated generation of recombinant influenza viruses that had hepatitis B virus (HBV) B cell epitopes in the NA stalk region as a dual vaccine candidate. MATERIALS AND

METHODS:

We used the WSH-HK reassortant helper virus for rescue of recombinant influenza virus containing HBV epitopes and reverse genetic protocol based on the use of micrococcal nuclease-treated virus cores for reconstitution of ribonucleoproteins.

RESULTS:

We successfully generated a chimeric influenza viruses which contained 22 amino acid peptides in the stalk region derived from the surface and pre-surface protein HBV. The growth kinetics of the recombinant viruses was investigated after infection of Madin-Darby canine kidney (MDCK) and Madin-Darby bovine kidney (MDBK) cells and the rIV-BVPreS virus showed higher titer than other viruses in MDCK cells. We also confirmed the presence of HBV epitopes in the chimeric viruses by enzyme-linked immunosorbent assay (ELISA) using anti-HBV polyclonal antibody. When the ratio of recombinant virus verse wild type virus was calculated by ELISA, recombinant viruses exhibited 2 fold higher values than the wild type virus.

CONCLUSION:

These results suggest that chimeric influenza virus which contained foreign antigens can be used as dual vaccine against both HBV and influenza viruses.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Orthomyxoviridae / Peptídeos / Vírus / Ensaio de Imunoadsorção Enzimática / Cinética / Vírus da Hepatite B / Sequência de Aminoácidos / Herpesvirus Cercopitecino 1 / Epitopos de Linfócito B / Influenza Humana Idioma: Inglês Revista: Clinical and Experimental Vaccine Research Ano de publicação: 2012 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Orthomyxoviridae / Peptídeos / Vírus / Ensaio de Imunoadsorção Enzimática / Cinética / Vírus da Hepatite B / Sequência de Aminoácidos / Herpesvirus Cercopitecino 1 / Epitopos de Linfócito B / Influenza Humana Idioma: Inglês Revista: Clinical and Experimental Vaccine Research Ano de publicação: 2012 Tipo de documento: Artigo