Effect of radiation dose variation on expression of caspase-3 in rat submandibular glands / 대한구강악안면방사선학회지
Korean Journal of Oral and Maxillofacial Radiology
;
: 7-15, 2006.
Artigo
em Coreano
| WPRIM
| ID: wpr-192596
ABSTRACT
PURPOSE:
To investigate the caspase-3 expression in the acinar and ductal cells of rat submandibular glands after the irradiation of various doses. MATERIALS ANDMETHODS:
The male Sprague-Dawley rats weighing approximately 250 gm were used for this study. The experimental group was irradiated with a single absorbed dose of 2, 5, 10, and 15 Gy on the head and neck region. The rats were sacrificed on the 1st, 3rd, 7th, 14th, 21st, and 28th day after irradiation. The specimens including the submandibular gland were sectioned and observed using histopathological and immunohistochemical methods.RESULTS:
The local destruction of the acinar and ductal cells and the karyopyknotic nuclei of the acinar cells were observed in the 2 Gy and 5 Gy irradiation groups later than in the 10 Gy and 15 Gy irradiation groups. And the expression of caspase-3 was prominent only in the ductal cells in the 2 Gy and 5 Gy irradiation groups.CONCLUSION:
This experiment suggests that radiation-induced apoptosis in the ductal cells of rat submandibular glands was induced by a low dose radiation associated with the activation of caspase-3 and radiation-induced necrosis was induced by a high dose radiation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Glândula Submandibular
/
Ratos Sprague-Dawley
/
Apoptose
/
Caspase 3
/
Células Acinares
/
Cabeça
/
Pescoço
/
Necrose
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Coreano
Revista:
Korean Journal of Oral and Maxillofacial Radiology
Ano de publicação:
2006
Tipo de documento:
Artigo
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