Expression of HSP70 and Its Relation with Other Cytokines in Human Middle Ear Effusion
Clinical and Experimental Otorhinolaryngology
;
: 18-23, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-192607
ABSTRACT
OBJECTIVES:
While other cytokines are known to be associated with otitis media with effusion (OME), the involvement of heat shock protein 70 (HSP70) in middle ear effusion (MEE) is unknown. This study was undertaken to investigate the possibility of there being a HSP70 expression in human MEE and to determine its potential role as a cytokine in OME.METHODS:
The levels of HSP70, tumor necrosis factor-alpha and interleukin-1beta were measured by enzyme-linked immunosorbent assay in the effusion of different groups of OME patient following collection of the MEE using our new collection system. The clinical characteristics of the OME patients and the MEE status were analyzed.RESULTS:
HSP70 was expressed in all the types of MEE. The mucous and seromucous effusions showed higher HSP70 levels than that of the serous effusion. The HSP70 level was correlated with the levels of tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta in the effusions. The positive correlations between HSP70, TNF-alpha and IL-1beta were statistically significant (P<0.05).CONCLUSION:
The highly elevated level of HSP70 in the seromucous and mucous effusions implicates this protein in the chronicity of OME.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Otite Média com Derrame
/
Ensaio de Imunoadsorção Enzimática
/
Citocinas
/
Interleucinas
/
Fator de Necrose Tumoral alfa
/
Proteínas de Choque Térmico HSP70
/
Orelha Média
/
Interleucina-1beta
/
Proteínas de Choque Térmico
Limite:
Humanos
Idioma:
Inglês
Revista:
Clinical and Experimental Otorhinolaryngology
Ano de publicação:
2010
Tipo de documento:
Artigo
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