Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses
The Korean Journal of Parasitology
; : 7-13, 2005.
Article
em En
| WPRIM
| ID: wpr-192618
Biblioteca responsável:
WPRO
ABSTRACT
The taxonomy of Acanthamoeba spp., an amphizoic amoeba which causes granulomatous amoebic encephalitis and chronic amoebic keratitis, has been revised many times. The taxonomic validity of some species has yet to be assessed. In this paper, we analyzed the morphological characteristics, nuclear 18s rDNA and mitochondrial 16s rDNA sequences and the Mt DNA RFLP of the type strains of four Acanthamoeba species, which had been previously designated as A. divionensis, A. parasidionensis, A. mauritaniensis, and A. rhysodes. The four isolates revealed characteristic group II morphology. They exhibited 18S rDNA sequence differences of 0.2-1.1% with each other, but more than 2% difference from the other compared reference strains. Four isolates formed a different clade from that of A. castellanii Castellani and the other strains in morphological group II on the phylogenetic tree. In light of these results, A. paradivionensis, A. divionensis, and A. mauritaniensis should be regarded as synonyms for A. rhysodes.
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WPRIM
Assunto principal:
Filogenia
/
Polimorfismo de Fragmento de Restrição
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DNA Mitocondrial
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DNA Ribossômico
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Acanthamoeba
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RNA Ribossômico 18S
Limite:
Animals
Idioma:
En
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2005
Tipo de documento:
Article