Flowers of Inula japonica Attenuate Inflammatory Responses
Immune Network
;
: 145-152, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-193640
ABSTRACT
BACKGROUND:
The flowers of Inula japonica (Inulae Flos) have long been used in traditional medicine for the treatment of inflammatory diseases. In the present study, we investigated the anti-inflammatory properties of Inulae Flos Extract (IFE).METHODS:
The anti-inflammatory effects of IFE against nitric oxide (NO), PGE2, TNF-alpha, and IL-6 release, as well as NF-kappa B and MAP kinase activation were evaluated in RAW 264.7 cells.RESULTS:
IFE inhibited the production of NO and the expression of inducible nitric oxide synthase (iNOS) in LPS-stimulated RAW264.7 cells. In addition, IFE reduced the release of pro-inflammatory cytokines, such as TNF-alpha and IL-6. Furthermore, IFE inhibited the NF-kappa B activation induced by LPS, which was associated with the abrogation of I kappa B-alpha degradation and subsequent decreases in nuclear p65 and p50 levels. Moreover, the phosphorylation of ERK, JNK, and p38 MAP kinases in LPS-stimulated RAW 264.7 cells was suppressed by IFE in a dose-dependent manner.CONCLUSION:
These results suggest that the anti-inflammation activities of IFE might be attributed to the inhibition of NO, iNOS and cytokine expression through the down-regulation of NF-kappa B activation via suppression of I kappa B alpha and MAP kinase phosphorylation in macrophages.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosforilação
/
Fosfotransferases
/
Dinoprostona
/
Regulação para Baixo
/
Citocinas
/
NF-kappa B
/
Interleucina-6
/
Fator de Necrose Tumoral alfa
/
Proteínas I-kappa B
/
Inula
Idioma:
Inglês
Revista:
Immune Network
Ano de publicação:
2010
Tipo de documento:
Artigo
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