Establishment of hydrochloric acid/lipopolysaccharide-induced pelvic inflammatory disease model
Journal of Veterinary Science
;
: 413-419, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-193784
ABSTRACT
Pelvic inflammatory disease (PID), which is one of the most problematic complications experienced by women with sexually transmitted diseases, frequently causes secondary infections after reproductive abnormalities in veterinary animals. Although the uterus is self-protective, it becomes fragile during periods or pregnancy. To investigate PID, bacteria or lipopolysaccharide (LPS) extracted from gram negative bacteria has been used to induce the disease in several animal models. However, when LPS is applied to the peritoneum, it often causes systemic sepsis leading to death and the PID was not consistently demonstrated. Hydrochloric acid (HCl) has been used to induce inflammation in the lungs and stomach but not tested for reproductive organs. In this study, we developed a PID model in mice by HCl and LPS sequential intracervical (i.c.) administration. The proinflammatory cytokines, interleukin (IL)-1β, IL-6 and tumor necrosis factor-α, were detected in the mouse uterus by western blot analysis and cytokine enzyme-linked immunosorbent assay after HCl (25 mg/kg) administration i.c. followed by four LPS (50 mg/kg) treatments. Moreover, mice exhibited increased infiltration of neutrophils in the endometrium and epithelial layer. These results suggest that ic co-administration of HCl and LPS induces PID in mice. This new model may provide a consistent and reproducible PID model for future research.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peritônio
/
Estômago
/
Bactérias
/
Útero
/
Ensaio de Imunoadsorção Enzimática
/
Infecções Sexualmente Transmissíveis
/
Western Blotting
/
Citocinas
/
Doença Inflamatória Pélvica
/
Interleucinas
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Feminino
/
Humanos
/
Gravidez
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2016
Tipo de documento:
Artigo
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