Cloning of Danio rerio toll-like receptor 1 / 나학회지
Korean Leprosy Bulletin
;
: 3-14, 2005.
Artigo
em Coreano
| WPRIM
| ID: wpr-194545
ABSTRACT
Toll-like receptors (TLRs) play a central role of innate immunity as well as of enhancing the adaptive immunity. TLR1 interacts with TLR2 to recognize the mycobacterial 19-kD lipoprotein. Danio rerio genomic sequences predicted TLR family genes. Danio rerio TLR1 gene was cloned with RACE-PCR and RT-PCR methods to get more sights of immunity against mycobacterial infection such as leprosy. Cloned TLR1 showed insertion of 22 amino acid residues comparing to NCBI- predicted TLR1, and an open reading frame composing of deduced 795 amino acids. Toll-interlukin1 receptor domain and leucine-rich repeats were in the cloned TLR1 as were noted in NCBI-predicted TLR1. Cloned zebrafish TLR1 had high similarity to human TLR1 with 35% amino acid identities and 57% amino acid positivities across the entire sequence (E=1e-130). The cloned sequence might help to study the innate and adaptive immunity of mycobacterial infection.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peixe-Zebra
/
Fases de Leitura Aberta
/
Células Clonais
/
Clonagem de Organismos
/
Receptores Toll-Like
/
Receptor 1 Toll-Like
/
Imunidade Adaptativa
/
Aminoácidos
/
Imunidade Inata
/
Hanseníase
Limite:
Humanos
Idioma:
Coreano
Revista:
Korean Leprosy Bulletin
Ano de publicação:
2005
Tipo de documento:
Artigo
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