Expression of Apoptotic Genes in Mouse Preimplantation Embryo Development / 대한불임학회지
Korean Journal of Fertility and Sterility
;
: 77-84, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-194596
ABSTRACT
OBJECTIVES:
The aim of this study was to evaluate the influence of three different media on preimplatation embryo development and the expression of Bcl-2, Mcl-1, Bax, and Bok in mouse. MATERiALS ANDMETHODS:
Two-cell embryos were retrieved from iCR female mice (4 weeks old) at 48 hr after hCG injection and cultured in Ham's F-10, HTF, and G1.2 media. The developmental rate of 2-cell embryos was evaluated from 24 hr to 72 hr after culture. RT-PCR was performed for the detection of Bcl-2, Mcl-1, Bax, and Bok gene expression.RESULTS:
The rates of morula and blastocyst in HTF and G1.2 media (88%, 98.1%) were significantly higher than those in Ham's F-10 media (39.6%) at 48 hr. Likewise, the rates of hatching and hatched blastocyst in HTF and G1.2 media (21.9%, 52.9%) were higher than those in Ham's F-10 media (3.5%) at 72 hr. Bcl-2 and Bax mRNAs were highly detected in embryos cultured in Ham's F-10 when compared in embryos cultured in HTF and G1.2. in contrast, the expression of Mcl-1 and Bok was not significantly different.CONCLUSION:
These results show that HTF and G1.2 culture media increase the rate of blastocyst formation and stimulate Bcl-2 and Bax gene expression in mouse preimplantation embryos.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Blastocisto
/
RNA Mensageiro
/
Expressão Gênica
/
Meios de Cultura
/
Desenvolvimento Embrionário
/
Estruturas Embrionárias
/
Mórula
Limite:
Animais
/
Feminino
/
Humanos
/
Gravidez
Idioma:
Coreano
Revista:
Korean Journal of Fertility and Sterility
Ano de publicação:
2003
Tipo de documento:
Artigo
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