Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model
Journal of Veterinary Science
;
: 439-442, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-194852
ABSTRACT
The ferret is an established animal model of influenza virus infection. Although viral replication in the upper respiratory tract is usually measured with consecutively collected nasal washes, daily evaluation of viral replication in the lung is limited because a large numbers of ferrets need to be sacrificed at consecutive time points. To overcome this limitation, we performed a virus quantification assay using bronchoalveolar lavage (BAL) fluid. This non-invasive BAL technique allows consecutive quantification of virus replication in the lungs of living ferrets. Our method can be used for the longitudinal evaluation of virus tropism in the lower respiratory tract.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sistema Respiratório
/
Replicação Viral
/
Infecções por Orthomyxoviridae
/
Lavagem Broncoalveolar
/
Modelos Animais de Doenças
/
Vírus da Influenza A Subtipo H3N2
/
Furões
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2014
Tipo de documento:
Artigo
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