The Effect of Vasoactive Intestinal Peptide on Cord Blood CD34 (+) Cells / 대한소아혈액종양학회지
Korean Journal of Pediatric Hematology-Oncology
;
: 262-268, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-194907
ABSTRACT
PURPOSE:
We investigated the expression of vasoactive intestinal peptide (VIP), VIP receptor 1 (VPAC1), VIP receptor 2 (VPAC2) genes in the human umbilical cord blood CD34 cells, and the ability of VIP to stimulate human primitive as well as monopotent hematopoietic progenitors.METHODS:
We isolated RNA from umbilical cord blood CD34 cells, and then performed RT-PCR, and sequencing. The umbilical cord blood CD34 cells were cultured with the various concentrations of VIP for burst-forming unit of erythrocyte (BFU-E), colony-forming unit of granulocyte/monocyte (CFU-GM), colony-forming unit of graulocyte/erythrocyte/monocyte/megakaryocyte (CFU-GEMM), and colony-forming unit of megakaryocyte (CFU-Mk).RESULTS:
The RNA coding for VPAC1 was detected in human umbilical cord blood CD34 cells. VIP significantly stimulated the growth of CFU-GEMM and CFU-Mk.CONCLUSION:
The present results suggest that VIP is an important neuropeptide in the early proliferation of human primitive as well as megakaryocyte progenitors.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células-Tronco
/
Neuropeptídeos
/
RNA
/
Megacariócitos
/
Peptídeo Intestinal Vasoativo
/
Receptores de Peptídeo Intestinal Vasoativo
/
Células Progenitoras Mieloides
/
Eritrócitos
/
Células Progenitoras de Megacariócitos
/
Sangue Fetal
Limite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Pediatric Hematology-Oncology
Ano de publicação:
2003
Tipo de documento:
Artigo
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