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Determining the Optimal Time for Peripheral Blood Stem Cell Harvest by Detecting Immature Cells using Hematology Analyzer, SE-9000TM IMI Channel / 대한임상병리학회지
Korean Journal of Clinical Pathology ; : 252-257, 1999.
Artigo em Coreano | WPRIM | ID: wpr-195437
ABSTRACT

BACKGROUND:

A key to successful peripheral blood stem cell transplantation is to harvest a sufficient amount of hematopoietic stem cells. A method of quickly detecting hematopoietic stem cells in peripheral blood with simple procedures using the SE-9000TM IMI channel (TOA Medical Electronics Co., Ltd., Kobe, Japan) was developed. In this study, usefulness of determining the optimal time for peripheral blood stem cell harvest using IMI channel was investigated.

METHODS:

Seventy nine peripheral blood stem cell collections were performed from thirteen patients with hematologic malignancy and nineteen patients with solid organ malignancy. In 13 cases, G-CSF was administrered following chemotherapy. In 19 cases only G-CSF was used to mobilize the peripheral blood stem cells. The counts of leukocytes, mononuclear cells, CD34 positive cells, and IMI in peripheral blood and leukapheresis products were determined.

RESULTS:

The CD34 positive cell count in harvested PBSC showed positive correlation with leukocyte cell, mononuclear cell, CD34 positive cell, and IMI in peripheral blood, with correlation coefficients of 0.48, 0.27, 0.63, 0.66, respectively. Positive correlation was presented between IMI and CD34 positive cell in peripheral blood and harvested PBSC, with a correlation coefficient, 0.83 and 0.74, respectively.

CONCLUSIONS:

As the SE-9000TM enables determination of the number of PBSC easily and rapidly, within approximately 85 seconds, whereas CD34 assays is expensive and needs skilled operator, the measurement of IMI positive cells is clinically useful for monitoring the peripheral blood stem cell mobilization.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Células-Tronco / Células-Tronco Hematopoéticas / Leucócitos Mononucleares / Contagem de Células / Fator Estimulador de Colônias de Granulócitos / Leucaférese / Neoplasias Hematológicas / Mobilização de Células-Tronco Hematopoéticas / Transplante de Células-Tronco de Sangue Periférico / Tratamento Farmacológico Limite: Humanos Idioma: Coreano Revista: Korean Journal of Clinical Pathology Ano de publicação: 1999 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Células-Tronco / Células-Tronco Hematopoéticas / Leucócitos Mononucleares / Contagem de Células / Fator Estimulador de Colônias de Granulócitos / Leucaférese / Neoplasias Hematológicas / Mobilização de Células-Tronco Hematopoéticas / Transplante de Células-Tronco de Sangue Periférico / Tratamento Farmacológico Limite: Humanos Idioma: Coreano Revista: Korean Journal of Clinical Pathology Ano de publicação: 1999 Tipo de documento: Artigo