Decreased Expression and Induced Nucleocytoplasmic Translocation of Pancreatic and Duodenal Homeobox 1 in INS-1 Cells Exposed to High Glucose and Palmitate
Diabetes & Metabolism Journal
;
: 65-71, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-196222
ABSTRACT
BACKGROUND:
Type 2 diabetes mellitus (T2DM) is often accompanied by increased levels of circulating fatty acid. Elevations in fatty acids and glucose for prolonged periods of time have been suggested to cause progressive dysfunction or apoptosis of pancreatic beta cells in T2DM. However, the precise mechanism of this adverse effect is not well understood.METHODS:
INS-1 rat-derived insulin-secreting cells were exposed to 30 mM glucose and 0.25 mM palmitate for 48 hours.RESULTS:
The production of reactive oxygen species increased significantly. Pancreatic and duodenal homeobox 1 (Pdx1) expression was down-regulated, as assessed by reverse transcription-polymerase chain reaction and Western blot analyses. The promoter activities of insulin and Pdx1 were also diminished. Of note, there was nucleocytoplasmic translocation of Pdx1, which was partially prevented by treatment with an antioxidant, N-acetyl-L-cysteine.CONCLUSION:
Our data suggest that prolonged exposure of beta cells to elevated levels of glucose and palmitate negatively affects Pdx1 expression via oxidative stress.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Genes Homeobox
/
Western Blotting
/
Espécies Reativas de Oxigênio
/
Apoptose
/
Estresse Oxidativo
/
Diabetes Mellitus Tipo 2
/
Células Secretoras de Insulina
/
Ácidos Graxos
/
Glucose
/
Insulina
Idioma:
Inglês
Revista:
Diabetes & Metabolism Journal
Ano de publicação:
2011
Tipo de documento:
Artigo
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