Analysis of 205 Cases of Parasite Infection Confirmed in Clinical Specimens / 대한임상미생물학회지

ABSTRACT

BACKGROUND: We analysed 205 cases of parasitic infection that were confirmed at Dept. of Parasitology, Hanyang University, College of Medicine from January 1986 to December 2003. METHODS: Parasitic worms were observed on gross examination or light microscope after treatment with lactophenol. Stool was examined with formalin-ether method for detection of eggs or protozoal cysts and scotch tape method was used for E. vermicularis eggs. In case of S. stercoralis, stool sample was incubated at 26degrees C for 48 to 72 h to confirm filariform larvae. Commercial ELISA kit for T. canis and ELISA test with hydatid cystic fluid from a patient were evaluated. Tissues were stained H&E after 10% formalin fixation and observed under the light microscope. RESULTS: There were detected 31 species of parasite among 205 specimens. Nematodes of 84 cases (41.5%), Anisakis sp., P. decipiens, E. vermicularis and T. callipaeda, visceral larva migrans, T. trichiura, A. lumbricoides, S. stercoralis, cutaneous larva migrans, Mammomonogamus sp. were observed. Cestodes of 41 cases (21.0%), D. latum, sparganum, T. saginata, cysticercus cellulosae, hydatid cyst and trematodes of 34 cases (16.6%), C. sinensis, M. yokogawai, P. westermani and N. seoulense were noted. Protozoa of 34 cases (16.6%), E. histolytica, E. dispar, T. vaginalis, Plasmodium sp. G. lamblia, E. coli, E. nana, P. carinii and arthropoda of 11 cases (5.4%), I. nipponensis, P. pubis, T. floricolum and Culex sp. larva were classified. CONCLUSIONS: Food-borne parasitic infections were distinctly noted in this analysis. And raw food or water are important as a source of parasitic infection in the future.